A common fix is to pilot-screen medium-sized perturbation libraries with identification of novel phenotype classes by visual inspection. Morphology space occupied by negative controls is initially learned without supervision, using a one-class-support-vector machine. The resulting classifier is then applied to the full screening data to identify novel phenotype classes based on their degree of morphological deviation from negative controls.
High-Throughput Screening / High-Content Screening | Hamamatsu Photonics
This method accurately detected several distinct mitotic phenotype classes, indicating feasibility of fully automated screening independent of user training. In ongoing experiments, we use the outlier detection method to identify new mitotic chromosome morphology regulators. With this approach it is possible to exploit 3D information and SPIM without running into a computational bottleneck. Bickle, Dorval and Montoya were more than pleased with the high quality and innovation in Dresden presentations.
It is more than a matter of cost, but also a way to share experience between the actors of the HCS field that is still highly challenging. These two packages combined allow robust segmentation of iPSCs solely on phase-contrast single-channel images and enable live imaging data to be easily integrated to endpoint data sets while retaining the dynamics of cellular responses.
Booij et al. A collaborative team from The Netherlands and Hungary describe a high-content screening platform that uses invasive human prostate cancer cells cultured in 3D in standard well assay plates to study the activity of potential therapeutic small molecules and antibody biologics.
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They processed 3D image data to measure more than phenotypic parameters; used multiparametric analysis to evaluate the effect of compounds on tissue morphology; then measured the activity and selectivity of inhibitors. Corresponding author Leo S. Price likens the task of performing high-content 3D screening experiments to that of the astronauts and NASA land team in the movie Apollo 13 , when tasked with finding a way to power up the command module for safe passage back to earth.
Using image analysis, we can separate those out. By analyzing phenotypes in detail and getting maximum information out of the images, you gain much more insight into what those drugs are doing and can classify drugs based on the responses that they induce.
10 Tips for Successful High Content Screening (HCS) Assays
Price wants SLAS Webinar participants to understand that 3D culture can be applied to pretty much any solid tissue and certainly to solid tumors. The conference was a good size and maybe the fact that there was limited room in the facility meant interaction was easier and more intense. It was great for getting to know people and talking to people.
Extended coffee and lunch breaks held near the posters allowed time for meaningful discussions with authors and others. It was great experience for them.
High Content Screening & Analysis
Completing the knowledge exchange, conference attendees also were able to visit 12 tabletop exhibits and attend tutorial sessions presented by PerkinElmer, Molecular Devices, Wako Automation and Thermo Fisher Scientific. We could have had many more vendors, but we did not have the space. In the tutorial sessions that were held before and after the event, vendors had the opportunity to sit together with users to discuss details and see how to get the most out of their data and instruments.
Holding the event at the Max Planck Institute also allowed attendees a first-hand look at some of its labs, and Bickle himself escorted several groups through his areas in the MPI Technology Development Studio.
It enables to retrieve a sharp focus without time consuming vertical positioning adjustment of the samples. This enables unrivaled screening speed. An additional advantage is that focalization can be performed after acquisition, reducing the risk of losing an important set of data due to bad vertical adjustment. With multiple measurements in a single acquisition, short screening time and low running costs, DHM is a very attractive solution.
High Content Screening. Scanning of wells plate. Increase efficiency of your screenings Measure more data points per day Perform non-invasive time-lapse screening Perform assays too complex for conventional systems Perform easier cell segmentation for faster and easier data analysis Make repeated measurements without washout since each well can be its own control Analyze simultaneously many different cellular processes with a single system.
Fluorescence imaging Epifluorescence Confocal Microscopy.
It enables bias-free screening, and long time-lapse experiments. An additional advantage is that focalization can be performed after acquisition, reducing the risk of losing an important set of data as a consequence of bad vertical adjustment. This key feature enables also to measure cells in 3D gels. Hit validation Dose response Time-lapse measure kinetics and time of onset. Biological Imaging.
Introduction to phase imaging in biology Biological Imaging. Electroneutral Transporter Monitoring Biological Imaging. Hematological Clinical Parameters Biological Imaging. Living Cells.